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... al idea: "At the same time, I support our efforts to explore brand new connection methods. I have noticed that in recent years, there is a split-intein system modified based on the principle of ’protein splicing’, which theoretically can achieve seamless connection of the C-terminus and N-terminus of proteins. If we can modify its recognition sequences and reaction conditions to connect two independent proteins, it will be a revolutionary breakthrough. But this requires a lot of protein engineer ...

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