MTL - The Science Fiction World of Xueba-Chapter 401 Target RNA

Pang Xuelinton paused and continued: "When the Hive Lab virus leaked, I was in a hurry. I injected a sample of the new virus directly into my body, and I did not have time to copy the relevant gene sequence of the new virus from the Hive Lab. , So now it ’s very difficult to re-develo that virus, and there is another problem ... "

"what is the problem?"

"The Kempten base laboratory is relatively simple, and we lack some high-end experimental equipment and equipment. For example, we now want to determine the dynamic characteristics of proteins and need dynamic APT, which is the so-called dynamic atom probe chromatography. Through it, we The dynamics of protein molecules and RNA molecules can be observed. "

Albert groaned for a moment and said, "You make a list of the supplies you need, and I'll send someone to help you get all of them."

Pang Xuelin said with a satisfied smile on his face, "Mr. Albert, please trouble you."

Next, Pang Xuelin cooperated with scientists brought by Albert Wesker and let them collect relevant blood and biological samples from themselves.

He even called Alice and asked her to collect samples with the umbrella company.

Today, because of Pang Xuelin's appearance, Alice is no longer the focus of the umbrella company, and Albert did not express any opinion on Alice's appearance at the Kempten base.

After all these tasks were completed, Albert Wesker declined the invitation of Pang Xuelin to have dinner, and hurriedly left by plane.

Looking at the helicopter that the umbrella company was leaving, Pang Xuelin said to Alice, who was next to him: "Alice, get ready. After the umbrella company equipment is in place, you go to Hill Military Base."

"it is good!"

Alice nodded.

Two days later, the umbrella company sent a dynamic atom probe tomograph and various cutting-edge experimental instruments and consumables.

Spruce, Berkeley, Huang Yu, and others were stunned. Many of the experimental equipment inside were unique to the umbrella company. They had never seen it outside.

Even Pang Xuelin looked around the dynamic atom probe chromatography for a long time. If this thing is put into modern society, it will be a big killer in terms of biology and materials science.

It's just a pity that his main energy now is to study how to fight the T virus, and he has no time to learn the technology of APT.

A week later, Alice led an elite team of more than two hundred people, swept the Hill base at the expense of more than twenty people, and obtained a large amount of weapons and ammunition from the Hill base.

In addition to rifles, machine guns, armored vehicles and various artillery, Alice also brought back three tilt-rotor helicopters.

In this way, the long-distance transportation of the Kempten base is no longer dependent only on the fleet of the umbrella company, which is of great significance for the future development of the Kempten base.

In addition, Alice did not disappoint Pang Xuelin, and she actually obtained three tactical nuclear warheads at Hill Air Force Base.

However, instead of returning the nuclear warheads to the Kempten base, Alice hid in a valley twenty kilometers away from the Kempten base.

Only Pang Xuelin, Alice, Claire, and Jill knew this. The three nuclear warheads had a small equivalent weight, only equivalent to the power of 20,000 tons of TNT, and the weight of a single piece was only about 50 kilograms.

Pang Xuelin was able to walk in the mountains with two nuclear bombs.

The secret bases of the umbrella company are basically located underground. If nothing can be done, or if Spencer pressures the Kempten base after realizing that Pang Xuelin has been perfunctory, these three nuclear warheads are enough for Pang Xuelin to destroy Underground base in Bince.

Late at night, after confirming that the three nuclear bombs were hidden, Pang Xuelin and Alice returned to Kempten base silently.

"Okay, it's getting late, you can go back and rest early."

Pang Xuelin is preparing to bid farewell to Alice and return to her room to rest.

Alice suddenly said, "Wait ..."

"what happened?"

Pang Xuelin turned around and looked at Alice.

Alice said, "Pang, when will we be able to attack the umbrella company?"

Pang Xuelin froze slightly and said, "Isn't it okay, wait until I have developed an antidote against the T virus?"

Alice shook her head and said, "I'm afraid I can't wait that long."

"why?"

Pang Xuelin frowned.

Alice said: "Spencer wants to destroy all human beings to build his super empire. It has been almost a year since the T virus outbreak. At present, the surviving humans in the world add up to ten million There must be. In the next few years, I am afraid that even if we kill Spencer, there will be few humans on the planet. "

Pang Xuelin said: "What are you going to do?"

Alice said: "One year, within one year, if you can get an antidote for the T virus, then we will continue to cooperate. If you are not sure, I will go to Spencer with a nuclear bomb."

Pang Xuelin looked at Alice quietly, he could feel the other's determination.

Pang Xuelin groaned for a moment and said, "Okay, then I try my best."

Alice nodded slightly, stopped talking, and turned away.

Pang Xuelin looked helpless at Alice's back.

Although he flew Alice to the Kempten base, and the two have been together for half a year, he could feel that Alice did not have complete trust in herself.

However, this is also no way out, Pang Xuelin is still in trouble with the umbrella company.

If it wasn't for Spencer's interest in Pang Xuelin's genetic optimizer, and if she didn't start with Alice, I'd be afraid that Alice would have gone a long way.

...

Alice's words put pressure on Pang Xuelin. In the following time, he devoted all his energy to the research on antiviral drugs for T virus.

This time, it was Turners, Ashford and Berkeley who were shocked.

Although Pang Xuelin followed them to study the T virus, most of the time he played an assistant role, and rarely voluntarily expressed personal opinions on academic issues.

But when he really began to dominate the operation of the laboratory, he soon showed the strength of an academic bull.

There are more than 30 people in the laboratory. In addition to Pang Xuelin, Spruce, Berkeley, Huang Yu, and Ashford, the rest are doctoral students with a certain academic background like Xiaodongbei Or research assistant.

In the first three months of the study.

Pang Xuelin first asked Xiaobeidong to analyze the structure and various data of various proteins and non-coding RNA in the cell through equipment such as cryo-electron microscopy, atomic probe chromatography, and nuclear magnetic resonance.

After obtaining these data, Pang Xuelin began to establish the dynamics and biological function models of the structure and dynamic characteristics of proteins and non-coding RNA through mathematical methods controlled by MOSS, and asked MOSS to write simulation software for proteins and non-coding RNA.

This work is tedious and complicated, but Pang Xuelin has relied on his strong mathematical skills and the supercomputing power of quantum computers to complete the writing of software.

When he applied this software to actual work, he immediately spoiled them.

With this software, scientists can actively construct some new RNA molecules and protein molecules, and then use the software to simulate the biological functions of these molecules.

As a result, they soon discovered that the software not only had very high simulation accuracy, but also predicted the existence of some undiscovered protein molecules and RNA molecules.

Moreover, Pang Xuelin used this software to build a biodynamic model of the T virus, which perfected the picture of the T virus invading the human body.

First, the T virus binds to specific receptors of the host cell through its surface glycoprotein, and then the viral envelope and the plasma membrane (ie, via the pH-independent pathway) or the endosome membrane with an acidic environment (PH-dependent pathway) After fusion, the viral ribonucleoprotein complex (RNP) is released into the cytoplasm, and each mRNA is synthesized during the transcription process, and the full-length antisense genomic RNA is generated by replication to make it a template for viral genomic RNA.

The newly synthesized RNP complex is assembled with viral structural proteins on the plasma membrane or Golgi membrane, and then the newly synthesized progeny virus is released.

The progeny virus grows and reproduces inside the cell, and then launches a large-scale attack on surrounding cells.

In addition, T virus also has a magical property. The RNA in its body can not only use normal human cells for reproduction, but also form a linkage effect with some non-coding RNAs in the human body, which can change the growth and metabolism rate of human cells on a large scale.

What Pang Xuelin has to do is to find a non-coding RNA that can interfere with the T virus RNA, so that the T virus is silent. Even if it infects the human body, because the gene cannot be expressed, it cannot be used in a large area in the human body. Breed.

...

Time passes day by day, and unconsciously, half a year is fleeting.

On this day, Pang Xuelin just came to the laboratory, and Spruce, who was on duty, happily found Pang Xuelin, and laughed, "Professor Pang, have we found the target RNA?"

"I found it, what group?"

Pang Xuelin's eyes brightened, some surprise.

Before using the protein and RNA molecular biodynamic function simulation software developed by Pang Xuelin, Pang Xuelin had written a special RNA with the T virus gene in a state of silence.

They then screened thousands of RNAs in human cells to find more than two hundred RNAs.

The next step is to experiment in groups.

This kind of work is cumbersome, basically basically repeating the previous work.

Pang Xuelin didn't take the initiative to participate. He has planned the follow-up research route, waiting only for the day when the target RNA is found.

Only during this time, the laboratory has tested hundreds of non-coding RNAs and has never achieved the desired effect.

Pang Xuelin wondered if he had gone the wrong way. If the agreed time of the year is up, Alice really wants to leave the town of Kempten and embark on a path of direct confrontation with the umbrella company. Then Pang Xuelin has no good way to stop other side.

Spruce said: "It is group 112. After we injected this RNA active extract into cells, we observed a significant decrease in the T virus content in the cells through dynamic APT, and even stopped the synthesis of RNP complexes. . "

Pang Xuelin nodded and smiled. "This is good news. Have you observed how the non-coding RNA of 112 can achieve gene silencing of T virus RNA?"

Spruce said: "We found that after RNA 112 enters the cell, the intracellular RNA-protein linkage enables deacetylation and methylation of lysine residues in the N-terminal domain of histones. Modification. This process is catalyzed by methyltransferases. Modifications can be monovalent, divalent, and trivalent methylation. T virus RNA can form heterochromatin with proteins that are bound by methylation-modified histones. In the above process, in addition to the deacetylation and methylation of the N-terminal tail domain of some histones, sometimes it is also necessary to lysine or arginine residues on other N-terminal tail domains of histones. The corresponding acetylation modification is performed, and the end result of this modification is that the gene silence of the corresponding segment of the T virus loses transcriptional activity ... "

Pang Xuelin groaned for a moment and said, "Look at Dr. Ashford, Professor Huang Yu, and Professor Berkeley. We will hold a closed-door meeting to study the follow-up work plan."

"it is good!"

Spruce nodded.

Finding the target RNA is just ~ www.novelbuddy.com ~ There is more important work to do next.

Pang Xuelin wants more than just making humans immune to the T virus. What he wants to do is how to use this target RNA to make a new type of airborne virus, which is basically equivalent to a kind of Harmful endogenous retroviruses, but in the body of zombies and T virus infected people, they can inactivate T virus in their body instantly.

In this case, when dealing with Spencer and his subordinates, they lost all the super powers conferred by the T virus, so Spencer was nothing more than a bunch of chickens in front of Pang Xuelin.

This kind of work requires the concerted efforts of Ashford, Berkeley, and Huang Yu.

Sure enough, after learning that the target RNA was found, Ashford and Berkeley both showed joy on their faces.

The discovery of the target RNA allowed them to see a ray of hope in this dark age.

But using the target RNA to assemble a new virus is also not a simple task. Pang Xuelin discussed with Spruce, Ashford, Berkeley, and Huang Yu for a full week before setting up the target virus.

Next, they combined this RNA with an adenovirus (AAV, previously mentioned, this virus is harmless to humans, and many people have it in the body. Scientists often use adenovirus to make vaccines), and then use The new gene editing tool adds some genes to the adenovirus, making this new virus capable of air transmission.

By this time, it was less than a month away from the agreement between Pang Xuelin and Alice.